In the imaging experiments, the sample was spray coated by using a TLC glass reagent sprayer (Sigma).
As another procedure, before the ninhydrin reagent was sprayed, diluted perchloric acid was sprayed upon the plate, and the plate was heated.
After UV-detectable (λ=253.7nm) spot was checked, the ninhydrin reagent was sprayed upon the TLC plate.
NH3-2-propanol 5 : 15 : 80 and ninhydrin reagent for spraying.
The residue was analyzed by TLC with silica gel G as adsorbents, 10% H3PO4·12MoO3 (phosphomolybdic acid hydrate) in 95% EtOH as detection reagent for spraying, followed by heating the plate to develop the colors, and solvent A, CHCl3 CH3OH (8-1), and solvent B, EtOAc-acetone (2.5-2) asolventnt systems, respectively, for development of sugars.
After 10 minutes each of plates was sprayed with a different spraying reagent for the identification of appropriate phytochemical.
A glass sprayer was used to spray reagent on the plates to locate the position of the spot of the analyte.
A glass sprayer (Borosil, India) was used to spray reagent on the plates to locate the positions of the spots of analytes.
After developing the plate was air dried examined on day light, UV-light (365 nm) and sprayed with spray reagent composed of 5 mL anisaldehyde, 10 mL glacial acetic acid mixed with 85 mL methanol and concentrated H2SO4.
A major product, N-acetyldiacetonamine (2), was obtained by preparative TLC (134.2 mg, 89% yield) as a pinkish purple-colored spot upon spraying with p-anisaldehyde-sulfuric acid spray reagent followed by heating.
