Sentence examples for reagent setup from inspiring English sources

Receptor-overexpressed cells (see "Reagent setup" section).

Dissolve Protein L (see "Reagent setup" section) in 0.2 mol/L phosphate buffer (pH 7.4) at a concentration of 2 mg/mL.

Linear 22-kDa polyethylenimine (see reagent setup) (Polyplus transfection, cat. no. 201-10G).

To avoid contamination, DNA extraction, reagent setup, PCR, and electrophoresis were performed in separate rooms.

To minimize contamination, DNA extraction, reagent setup, amplification, and agarose gel electrophoresis were performed in separate rooms.

To avoid contamination, we performed DNA extraction, reagent setup, amplification, and agarose gel electrophoresis in separate rooms and included negative controls (distilled water) were in each amplification.

Other variables facing multicolor assay depend on antibodies and fluorochromes used, fixation and permeabilization reagents, instrument setup, data acquisition and analysis, reporting of results, internal quality control, external quality assessment, and flow sorting [ 53] (Table 1).

All PCRs were performed in a unidirectional workflow through dedicated suites for reagent preparation, PCR setup, and amplification.

Field samples from S. Salar (n = 98), O. mykiss (n = 97) and O. kisutch (n = 82) were obtained from different farms located in the south of Chile, and parallel assays were run under the same RT-qPCR conditions (reagent mix, software setup, and data analysis).

During the setup all reagents and the 0.2 ml thin-wall tubes were kept in bench-top coolers.

To minimize risk for contamination, pipettor sets were dedicated to specific functions, i.e., reagent dispensing, template isolation, PCR setup, and template handling.