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Sequence of low level steps required to add the contents of a reagent placed in the second reagent position of the first cassette to the first reaction vessel.
The sample was mixed with the LAL/substrate reagent, placed in an incubating plate reader, and monitored over time for the appearance of a yellow color, according to the instructions of the manufacturer.
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We also present a preliminary concept approach for developing a reagent placing device with simple and inexpensive features.
For immunostaining, the grids were floated on drops of immune reagents placed on a sheet of parafilm.
Alternatively, the migration of two different populations of cells can be evaluated from the side wells, experiencing identical concentration gradients generated by reagents placed in the central well.
Single embryos, single blastomeres or ICMs were delivered to dry droplets of denaturing reagents placed on PCR tube lids (LysoDots), briefly heated and dried again and stored at -20°C, as detailed in reference [ 3].
Reagents placed in solution reservoirs were pumped though plastic hoses by means of a Masterflex L/S microprocessor-controlled peristaltic pump (Cole-Parmer, Vernon Hills, IL) to a quartz fast-flow mixing chamber flat cell (Type WG-804, 10 mm width, Wilmad Glass Co., Buena, NJ).
The titrant (reagent) is placed in a buret and is added stepwise to the assayed substance.
We evaluated BACs in bats using ethanol-sensitive reagent pads placed in the mouth for 30 s (ALCO-Screen®, Chematics, USA).
Briefly, cells were vortexed for 15 seconds in cytoplasmic extraction reagent and placed on ice for 1 min.
A 1.5 mL of the culture supernatant was mixed with 3.0 mL DNS reagent and placed in boiling water for 5 min.
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