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The disease control centers said laboratories across the country should review the reagent package insert information to verify that the correct calculations were being used.
Source: Binax NOW® reagent package insert, 2007 page 6.
D-dimer was measured with microlatex immunoturbility assay (Simens Innovance D-Dimer Reagent package insert, Dade Behring, Marburg, Germany).
Protein concentration was determined using the Bio-Rad DC Protein Assay Reagent Package (Bio-Rad Laboratories, Inc., Hercules, CA, Cat. 500-0116). 500-0116
The RNA samples were labelled using the GeneChip One-Cycle Target Labeling and Control Reagent package (Affymetrix) and then hybridized to the Affymetrix GeneChip Porcine genome array according to the manufacturer's instructions.
For Affymetrix analyses, cRNA was synthesized using the One-Cycle Target Labeling and Control Reagent package (Affymetrix Inc., Santa Clara, CA) and the reaction was probed to the 430A mouse Affymetrix chip.
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Data on performance appraisal done by testing laboratory technologists based on the following characteristics in the check list: number of steps in the test procedure, clarity of kit instructions, kit and reagent packaging and labeling, and ease of performance, as used in the previous study, [ 10] were also obtained.
After measuring the quantity and quality of the RNA, biotin-labeled cRNAs were generated from 5 μg of each total RNA using a GeneChip® One-Cycle Target Labeling and Control Reagents package (Affymetrix) according to the manufacturer's protocol.
Briefly, the One-Cycle Target Labeling and Control Reagents package (Affymetrix, Santa Clara, CA, USA) was used to synthesize cDNA from total RNA spiked with prokaryotic Poly-A RNA as a control.
The price, however, depends on too many factors not related to SA (for example novelty of the reagent, demand, packaging, marketing issues) to be relied on as an objective measure of SA.
293 TN cell was stably transfected with the constructs and three packaging plasmids using Lipofectamine 2000 reagent (Invitrogen) to package lentivirus; and then pancreatic cells were infected with lentivirus with multiplicity of infection of 5. Clones with stable downregulated FRS2 α or Mcl-1 expression were selected with puromycin (1 μg ml−1).
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