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Cerium reagent: orange.
We observed similar rates of TOG-induced depolymerization using DIC instead of fluorescence to monitor microtubule length (black triangles), as well as using GTPγS instead of epothilone as a stabilizing reagent (orange diamonds, purple squares).
Ligation was carried out for 30 min at 37°C and amplification was done for 100 min at 37°C using the Duolink In Situ Detection Reagent Orange (Olink; 92007).
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Before adding the diluted ligation mixture (Olink, Duolink In Situ Detection Reagents Orange) and incubating the tissue sections for 30 min at 37°C the slides were washed twice in TBS-T for 5 min.
The in situ PLA experiments were performed using reagents and instructions found in commercially available kits from Sigma-Aldrich; Duolink® In Situ PLA® Probe Anti-Rabbit MINUS/PLUS (DUO92005/DUO92002), Duolink® In Situ PLA® Probe Anti-Mouse MINUS/PLUS (DUO92004/DUO92001) and Duolink® In Situ Detection Reagents Orange (DUO92007).
Folin-ciocalteu reagent, xylenol orange and N, N-dimethyl-4-nitrosoaniline were obtained from Merck, Mumbai, India.
Reagents Methyl Orange (MO), Evans Blue (EB), Remazol Brilliant Blue (RBB), Sinapic acid, Acetosyringone (3′,5′-dimethoxy-4′-hydroxyacetophenone), Syringaldehyde (3,5-dimethoxy-4-hydroxybenzaldehyde), violuric acid (5- Hydroxyimino -2,4,6 5- Hydroxyimino -2,4,6 5- Hydroxyimino -2,4,6chased from Sigma–Aldrich.
Prior to co-culture with macrophages, thymocytes were labeled with a fluorescence cell-tracker reagent (CellTracker™ Orange CMTMR, Molecular Probes).
Reticulocytes count was analyzed with Retic-COUNT (ThiazOrangeange) Reagent (BD biosciences).
Briefly, cells were incubated in a wash buffer containing 2 μM of oxidative-stress- (green) or 2 μM of superoxide-detection (orange) reagent kit.
Free acidity was determined by titration with 0.01 N sodium hydroxide (NaOH) with methyl orange reagent until the color of the solution became yellowish.
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