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System Biosciences released a proprietary reagent named ExoQuick that can be added to serum, conditioned cell media, or urine and is claimed to precipitate the exosomes [ 32].
In 6 sets of PK conditions 250 μL of each sample was treated: 1 with 250 μL of A TeSeE reagent (TeSeE purification kit) with 10 μg of PK and 5 different PK concentrations (10 μg, 15 μg, 20 μg, 25 μg, and 30 μg) in 250 μL of A TeSeE Sheep & Goat reagent, named A′′ reagent (TeSeE Sheep & Goat purification kit).
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All reagents named above were purchased from Sigma Aldrich, and a power supply Keithley Model 2420 SourceMeter was used to carry out the DC electrodeposition process.
(b) Reagent name and description are entered in the two edit boxes above the layout diagram.
This reagent was named CBMG, or more affectionately Palau'chlor (which is based on the name of the iconic pyrrole-imidazole natural product, palau'amine).
Because the actual inhibitory activity was found to be from minor contaminations unrelated to the assigned chemical structure, the reagents are named by an abbreviation of the vender name followed by the catalog number (or as 'in-house' preparation).
The reagent is now named GUALY's reagent.
Because the last step can be catalyzed by DTT instead of thioredoxin, we can measure MSRA activity using DTT and the chromogenic substrate dithio-bis-nitrobenzoic acid (DTNB, alternatively named Ellman's reagent).
Other examples of the contents in chemical patents are compounds, reagents and solvents [7]. Figure 1 shows the classes of named chemical entities mined by different systems as described by examples.
Pyrosequencing reactions were carried on PSQ HS96A instrument using PSQ HS96A SNP reagents and pyrosequencing SNP analysis software (Biotage AB, Uppsala, Sweden, now named PyroMark™Q96MD by Qiagen).
Immunohistochemistry was performed using the un-avidin-biotin complex technique named EnVision following the manufacturer's instructions with the reagents supplied together with the kit.
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