Currently, OPEN reagents include modules that recognize all 16 possible GNN triplets (i.e., DNA triplets beginning with G, followed by any nucleotide in the second and third positions), as well as several TNN triplets.
Measurements of serum cholesterol, TG, and HDL cholesterol were performed using Roche reagent on a Roche Module P autoanalyzer (Roche Diagnostics, Indianapolis, IN).
After reagents are loaded, the module is ready to receive radioactivity for radiosynthesis.
Roche reagents on a P module of Roche Modular (Basel, Switzerland) were used to assess fasting glucose, triglycerides, HDL-cholesterol and low-density lipoprotein cholesterol (LDL-cholesterol).
The assay for serum glucose was performed enzymatically at the Northwest Lipid Research Laboratories University off Washington), using Roche reagents on a Roche Module P Chemistry autoanalyzer (Roche Diagnostics Inc., Indianapolis, IN).
The low molecular weight column calibration kit, enhanced chemiluminescence (ECL) reagents and ECL glycoprotein detection module were from GE Healthcare (Amersham, UK).
Serum cystatin C concentrations were determined using the Siemens Diagnostics reagent on a Roche Hitachi P-Module analyzer (Siemens Diagnostics, Deerfield, IL).
Estimates of chromosomal sizes were performed with pulsed-field gel electrophoresis (PFGE) on a CHEF Mapper XA system using reagents provided in the GenePath Univeral Module (Bio-Rad).
After amplification, the samples are fragmented using Module 2 of the Axiom Reagent Kit.
Re-suspension and hybridization preparation of the sample is carried out using Module 2 of the Axiom Reagent Kit.
