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The reagent mixtures, activated by means of a high-energy planetary mill AGO-2, were annealed at 350, 450 and 550 °C in air.
Dilute reagent mixtures of ∼100 ppm of each fuel were prepared in bulk argon and shock heated to study the stable intermediates.
The high-pressure single pulse shock tube (HPST) at the University of Illinois at Chicago has been used to study the oxidation of toluene at reflected shock pressures from 22 to 550 bar and temperatures from 1210 to 1480 K. Experiments were performed for dilute stoichiometric, Φ= 1, and rich, Φ= 5, reagent mixtures.
The titanium reagent mixtures were centrifuged at 12,000 g for 10 min and supernatants measured at 410 nm absorbance.
For DNA damage assays, supercoiled pUC19 DNA (1 µg/µl) (New England Biolabs) was diluted (1/25) in the specified reagent mixtures (Figure 5 and S3A).
Similar analyses on NCL reactions with different reagent mixtures are shown in Figure 2d.
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After preparation of the mixture of the siRNA duplex solution and transfection reagent, the siRNA transfection medium and the siRNA transfection reagent mixture were added to the cells.
High EDA ratios in the reagent mixture lead to a predominance of cage products with high values of n, and vice versa.
Hydrodynamic forces were generated by circulating the reagent mixture in a closed loop.
The experimental design methodology was applied having in mind the optimization of the azo dye Orange II degradation using the Fenton's reagent (mixture of H2O2 and Fe2+).
In this device, chip 1 was used to prepare and pump the reagent mixture, whereas chip 3 was used for pumping the sample.
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