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AM and MLB contributed reagent material and analysis tools.
Recombinant antigen early secreted and activated target (ESAT) -6 kDa and M. tuberculosis H37Rv whole sonicate (MTBs) were provided through the NIH tuberculosis vaccine testing and reagent material contract (NO1-A1-40091) awarded to Colorado State University, USA.
Hence, we performed a ligand binding experiment using 8 replicates for each concentration of CTB applied so as to obtain highly quantitative and information-rich binding data with very little reagent material required.
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FY and EC contributed reagents, material, and analysis tool.
ZXW contributed reagents, materials and analysis tools.
HNL, NMH and ZTJ contributed the reagents, materials and analysis instruments.
JL contributed reagents, materials and analysis tools.
MAC contributed reagents, materials and analysis tools.
MPW contributed reagents, materials and analysis tools.
SC contributed reagents, materials and analysis tools.
Angela Floden, Robert Gaultney, Catherine Brissette and John Watt contributed reagents, materials and critical guidance.
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