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GP was involved in reagent generation and manuscript preparation.
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The Supplemental Experimental Procedures provides a list of all reagents, generation of transgenic lines, and detailed description of all techniques used in this study.
In D-ROM test, the concentration of cell hydroperoxides generated by oxidative attack on various organic substrates like proteins, lipids or DNA is measured, in presence of iron (produced by the R2 reagent), after generation of alkoxyl and peroxyl radicals which will in turn oxidize an alkyl-substituted aromatic amine (R1 reagent), thus transforming them into a pink derivate.
Traditional cyanation methods suffer from serious drawbacks, such as the use of highly toxic reagents, the generation of stoichiometric quantities of metal waste, the need for harsh conditions, and poor functional-group tolerance.
After the application of a luciferin generation reagent and subsequent luciferin detection reagent in each well, luminescence was read.
The procedure minimizes the use of toxic chemical derivatization reagents and the generation of hazardous waste.
Based on our first-generation reagent 4-APC, the design of 4-APEBA addressed four stringent issues in biomarker analysis.
First, we cross-checked 4-APEBA in the derivatization of aldehydes against first-generation reagent 4-APC.
Compared to the first-generation reagent 4-APC, 4-APEBA contains an extra bromophenethyl group which results in several benefits.
Compared to the first-generation reagent 4-APC, 4-APEBA displays similar fragmentation patterns of the adducts.
MP provided essential contributions to lipid binding and helped with the analysis, AMT provided essential reagents for antibody generation.
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