Sentence examples for reagent extraction from inspiring English sources
Total RNA was extracted by Trizol reagent extraction method with reference to the method on the kit.
In addition, the BTA™ lysis buffer mildly but efficiently extracts DNA from challenging substrates like tape, chewing gum, and cigarette butts and, as with bone and tooth, DNA from these lysates is purified using established PrepFiler™ reagent extraction protocols.
Total RNA was isolated using the TRIzol reagent extraction kit (Invitrogen), according to manufacturer's instructions.
Samples were subjected to Trizol LS reagent extraction and resuspended in 20 µl DEPC-treated water.
Specificity of the PCR products was determined by melting curve analysis and amplification products were resolved on a 1.5% agarose gel to confirm the correct size of the amplicons (92, 102 and 226 bp for TLR2, TLR4 and GAPDH respectively). A. baumannii ATCC 17978 LPS was purified from 10 mg of lyophilized bacterial cells using the Tri-Reagent extraction method as previously described [28].
All PCR steps were carried out in separate rooms (clean reagents, extraction, and amplification rooms).
This experiment, which adopted environmentally friendly reagent as extraction solvent, not only improved the extraction efficiency, but also avoided the environmental pollution caused by organic solvent.
Total RNA was extracted following the Trizol Reagent RNA extraction kit manual.
Reagent and extraction blanks were included in every batch.
None of the target analytes were identified in the reagent or extraction blanks.
Total RNAs were purified by three rounds of Trizol reagent (GIBCO/BRL) extraction before precipitation.
