Qualitative differences between laboratories could be explained by differences in thiol-reagent content of assay medium and by the method of homogenization.
After mixing of the reagent, the contents were sonicated for 5 20 min at an interval of 5 min.
The supernatant was mixed with 1 mL working OliGreen reagent and ODN content was estimated spectrofluorimetrically at 480 nm and a 520 nm excitation and emission, respectively [ 101, 102].
These organometallic reagents such as Grignard reagent have high content of energy that its reaction is very exothermic and release energy in the form of heat.
Quantitative reagent for protein contents was purchased from Bio-Rad (Hercules, CA).
We also report that syncytium formation is inhibited by reagents lowering the content and accessibility of PtdIns(4,5) P2, an important regulator of intracellular membrane remodelling.
Cells were stained using Cell Cycle Kit I reagents for DNA content and phospho-histone H3 staining (Thermo Fisher Scientific) according to the manufacturer's instructions.
The pellets were digested in an acetic-nitric reagent and the cellulose content was determined by anthrone according to the method described by Updegraff (1969).
After Blyscan dye reagent was added, the content of the tubes was homogenized for 30 min and centrifuged for 10 min (10,000 ×g).
In the present experiment, to establish a collection of BACs suitable for subsequent multiBAC-FISH comparative analyses, it was important to select clones showing specific hybridization in the absence of any reagent directed against repetitive content.
All protein samples were normalized to the total protein content (Bradford reagent; Bio-Rad Laboratories, HerCAles, CA, USA), and comparable loading, as well as protein transfer after blotting, was confirmed through detection of Actin as internal control.
