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cerium loading, temperature, reagent composition and water addition.
The description field can be used to make notes about the reagent composition and can include item or lot numbers to simplify reagent tracking and reordering.
The different assays involved gas diffusion through a Teflon planar membrane and were carried out after minor modifications in the manifold, namely reagent composition and total flow rate.
Our observations indicated that GC bias among samples was more substantial between sequencing runs than within runs, suggesting that such bias might have been introduced during the sequencing process, perhaps through slight differences in reagent composition and temperatures that led to differences in DNA denaturation and primer hybridization efficiency.
Details of the protocols, commercial reagents tested and custom reagent composition and stabilization procedures are described in supplementary data (Additional file 2).
Reactions were stopped precisely 15 or 30 min after ATP was added by adding 300 μL Lin Morales Reagent (see [ 30] for reagent composition) and vortexing.
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Controlled experiments were designed using varied temperatures and assistant reagent compositions to study their influences on the crystal phase and morphology of TiO2.
In addition, discrete control of each independent step of the two-step sequential reaction was demonstrated via selective reagent feedstock composition exposure of the top and/or bottom surface of an immobilized membrane/electrode system.
Reagent mixture composition shared by T and S PCR was 15 mM Tris HCl (pH 8.0), 50 mM KCl, 200 μM dNTP, 1% DMSO, 2.5 mM DTT, SYBR-Green I. Composition specific for the T PCR was 1.5 mM MgCl2, 1.5 Units AmpliTaq Gold DNA polymerase and 450 nM of each telomere specific primer.
Same experimental procedure was followed to prepare other samples, the changes being the variation in the treatment reagent and its composition.
The standard procedure [35] was followed using the Herzberg reagent to investigate fibre composition of samples from the ref-collection.
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