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This notion is further reaffirmed by data obtained from a limited analysis of gene expression in Hes1 transgenic tumor samples, showing expression of a combination of different Notch targets at varying levels in different tumors.
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Lack of P14ARF gene was reaffirmed by seminested P14ARF PCR (data not shown).
These data were reaffirmed by immunofluorescence as staining was diminished in aortic VSMCs infected with the lentivirus containing the shRNAmir directed against nestin (Additional file 1: Figure S1).
In case of negative P14ARF PCR, data were reaffirmed by seminested PCR: 3 μl of the first-run PCR mixture were transfered to a reaction cup and amplified using first-run P14ARF sense primer (Newcomb et al, 1999) and primer 5′-TCACCAAGAACCTGCGCACC-3′ (novel P14ARF antisense primer).
EID's impulse to swiftly provide urgent information free of charge to a global audience has been reaffirmed by several recent movements toward free access to scientific data.
The safety and the efficacy of the vaccine has been reaffirmed by numerous independent investigations, including a 2015 report based on data from over a million individuals which concluded the vaccine had a favourable safety profile.
The data corroborate previous studies showing PNV-induced BBB permeation in adult rats [ 3– 6], now reaffirmed by the extravasation of Evans blue injected peripherally.
"It's good to get reaffirmed by the people.
This resolution was reaffirmed by the General Assembly in 1977.
That message was reaffirmed by the British defense minister, Bob Ainsworth.
That status was reaffirmed by the I.R.S. as part of its settlement with the company.
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