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After the final wash step, ABTS-substrate (Sigma, ready-to-use solution) was added and the optical density was determined at 405 nm.
After three further washings, the coverslips were air-dried and mounted with DAPI (4',6-diamidino-2-phenylindole) from a ready-to-use solution, ProLong Gold Antifade Reagent (Molecular Probes).
In order to determine CRNK-16 vitality, cells were stained with 7-amino-actinomycin D (7-AAD), a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis (for details see Schmid et al. [49]).
Slides were then rinsed with tap water, air dried at room temperature, overlaid with DAPI (4', 6'-diamidino-2-phenylindole) ready-to-use solution (Molecular Probes, Montluçon, France) and examined using a Leica DM 2500 upright fluorescence microscope at 100× magnification with a FITC-rhodamine double band filter.
For direct fluorescence with DAPI (4',6'-diamidino-2-phenylindole) staining, cells were covered with 5 µM DAPI from a ready-to-use solution, "ProLong Gold Antifade Reagent" (Molecular Probes) and stained for 10 min in the dark prior to observation.
The pre-publication history for this paper can be accessed here: *rtu: ready-to-use solution.
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Although it does not provide the flexibility of common low-level design tools, it offers a set of comfortable, ready-to-use solutions for plant identification, loop-shaping or LQR/LQG-controller design and controller implementation.
Having reviewed already existing packages for the implementation of BCIs, we introduce our own solution which specifically considers the relevance of multi-subject handling for experimental and clinical trials, for example by implementing ready-to-use solutions for pseudo-/sham-neurofeedback.
Are you being sold ready-to-use solutions, or is the consultant fully focused on your agenda?
These ready-to-use solutions are maintained at 37°C until incubation.
QT provides similar ready-to-use solutions to a number of queueing systems although the mathematical expressions can be significantly more complex.
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