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They have been used extensively for the sequencing of the mouse and human genomes and the clones from these sequencing projects are available making almost the entire genomes available in ready sequenced clones.
Exiled from the commune, he reverts to "Lord of the Flies -like savagery in an MTV-ready sequence in which he conFlies -likety with hisavagery Game Boy.
Earlier this year, Monsanto, a large biotechnology firm, announced a rough-and-ready sequence of the rice genome, and an international consortium is now trying to refine it.
Nucleotide analysis was performed with using a d-Rhodamine Terminator Cycle Sequencing Ready Reaction-Sequencing Kit (Applied Biosystems) and an ABI377 Automated DNA Sequencer (Applied Biosystems).
These collections of selected BLASTN-ready sequences are referred to as the complete data sets, one for bacterial/archaeal sequences and one for eukaryote sequences.
The availability of BAC libraries from individual chromosomes greatly simplifies the construction of ready to sequence physical maps and the analysis of the complex allohexaploid wheat genome.
Forward and reverse cycle sequencing reactions were performed with the Big Dye Terminator Cycle Sequencing Ready Reaction DNA sequencing kit (Applied Biosystems, Inc., Foster City, CA, USA).
The library products were ready for sequencing via Illumina HiSeqTM 2000 or other sequencers when necessary.
The DNA template was denatured at 94°C for 4 min and stored in ice, ready for sequencing on a Perkin-Elmer 377 XL DNA sequencer (Applied Biosystems).
The amplified products were also purified using the DNA clean and concentrator (DCC) kit (Zymo research institute, South Africa) before being made ready for sequencing.
The library products were ready for sequencing analysis via Illumina HiSeq™ 2000.
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