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Direct sequencing of the PCR products was performed with an ABI BigDye terminator v3.1 sequencing Ready Reaction kit.
Sequencing reactions were as stated in the ABI Prism BigDye Terminator cycle Sequencing Ready Reaction Kit (Applied Biosystems), with 16 pmoles of primer and 6ul Big dye Terminator Ready Reaction Mix (Applied Biosystems) for a 20 ul reaction.
Sequencing was performed using BigDye Terminator Ready Reaction Mix.
Sequence analysis was performed with the BigDye Terminator Cycle Sequencing Ready Reaction Kit for both strands.
The amplicons were sequenced using the BigDye Terminator V2.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems).
Sequencing reaction was performed with a BigDye Terminator v3.1 Ready Reaction Cycle Sequencing kit (Applied Biosystems).
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Nucleotide analysis was performed with using a d-Rhodamine Terminator Cycle Sequencing Ready Reaction-Sequencing Kit (Applied Biosystems) and an ABI377 Automated DNA Sequencer (Applied Biosystems).
Fragments were labelled with the PRISM-Ready Reaction Dye Deoxy-TM Terminator Cycle Sequencing Kit (Applied Biosystems, Weiterstadt, Germany) and analysed in an ABI 310 analyzer.
Then, for each reaction, 5 µL of PCR product, 4 µL terminator-ready reaction mix, and 3 pmol of primer were added.
On two occasions during the November 1989 election, the ASC Ready-Reaction Force was used to disperse rioters.
AmpliTaq Taq polymerase and BigDye-Terminator-Cycle-Sequencing-Ready-Reaction kit were supplied by Perkin Elmer (San Jose, CA, USA).
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