PCR reactions for the marker analyses were carried out using α [P] dCTP (3000 ci/mmol) following the manufacturer's protocol (Multiplex-Ready Marker User Handbook, version 2.0).
Genomic map of target gene were established through three rounds of linkage analysis using genomic position-ready molecular markers (He et al. 2012).
The Barque-73/CPI71284-48 was typed with multiplex-ready SSR markers (Hayden, Nguyen and Chalmers, in preparation).
PH genotyped the multiplex-ready SSR markers for the Barque-73/CPI71284-48 populandon and co-developed an independent high-density map for this cross.
Nuclei were manually classified into the different cell types of the eye, which is possible because every cell undergoing differentiation can be unambiguously identified by its position and nuclear morphology without the need of cell-specific markers (Ready et al., 1976; Tomlinson, 1985; Tomlinson and Ready, 1987; Wolff and Ready, 1993).
Have a white board or chalk board with white board markers ready.
After cleaning the glass jar and removing the label, you can place the jar on a desk and use it to keep pencils, pens, or markers ready at hand.
The ready availability of dense marker maps and physical positions as well as genetic positions for markers facilitates incorporation of positional information.
Oh fuck, see that one wall, how it's all sort of crumbling on the edge, looking all urban and hot and ready for some fatty marker?
Therefore, a high frequency transformation system is essential, with a ready supply of selectable marker genes to allow multiple constructs to be expressed, and the ability to carry out targeted mutations (Kilaru and Steinberg, in 2015; Kilaru et al., 2015a; Schuster et al., 2015a,b; Mehrabi et al., 2015).
