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The library products were ready for sequencing via Illumina HiSeqTM 2000 or other sequencers when necessary.
The DNA template was denatured at 94°C for 4 min and stored in ice, ready for sequencing on a Perkin-Elmer 377 XL DNA sequencer (Applied Biosystems).
The amplified products were also purified using the DNA clean and concentrator (DCC) kit (Zymo research institute, South Africa) before being made ready for sequencing.
The library products were ready for sequencing analysis via Illumina HiSeq™ 2000.
Library products were ready for sequencing analyses via Illumina HiSeq™ 2000.
Target enriched libraries were stored at a stock concentration of 10 nM ready for sequencing.
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This product was then converted into a ready-for-sequencing library by PCR amplification followed by 1× AMPure XP beads purification.
Like, for sequencing your genome.
for sequencing.
Finally, the purified cDNA library was ready for deep sequencing (LC Sciences, Hangzhou, China).
However, the library preparation methods required to render immunoprecipitated DNA ready for HTS sequencing involve inefficient enzymatic steps and multiple purifications, each resulting in sample loss.
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