Then, the modified membrane disk was ready for sample extraction.
The ODS column temperature was ambient, and that of the CTH-packed reactor was 40°C. Figure 2 Schematic diagram of on-line-SPEn coupled to packed reactor FIA for the analysis of FA in pharmaceutical formulations: the system in initial position, ready for sample injection, derivatization and enrichment step (A); the back-flush elution and detection step (B) (S.V.: switching valve).
The pellet was then resuspended in 1 ml distilled water, ready for sample preparation and measurements.
When the spin column was ready for sample binding, 1 mL of centrifuged serum sample was added to the column and equilibrated at room temperature for 2 hr.
But Jerome Fuchs and Zach Hanna are also home and are already working in the lab, and they're ready for the samples to arrive (they should be here any day now).
When ready for processing, samples were equilibrated to room temperature and rehydrated into 1× phosphate-buffered saline (PBS) and then decalcified in a solution of 350 mM EDTA, 4% paraformaldehyde and 1× PBS.
It should be noted that some Olympic Venues were ready for use and sampling just one or two weeks before the Olympic Games, thus, the first sampling microbiological results were not satisfactory since inadequate flushing might have been carried out.
We were silent, inexpert, rather than sceptical, holding on to our tasting glasses ready for the next sample.
The separation column used was a Zorbax Rapid Resolution SB-C18 4.6 × 150 mm (Agilent, USA) with a binary solvent program (A = formic acid/MQ water (5:95); B = acetonitrile) that started at 95% A 5% B at injection, changed to 80%A 20%B at 9 minutes; 20%A 80%B at 18 minutes and held for 2 minutes before returning to 95%A 5%B ready for the next sample injection.
The sample preparation can either stand alone, meaning that it is used only to get a sample ready for analysis on another platform; or automated sample preparation can be integrated with an analytical platform, so that a sample is loaded, prepared, and analyzed within one device.
