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The cleaned data file was then submitted to recoding and transformations to create and calculate variables ready for analyses.
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The contents were then filtered and kept in acid-washed polythene bottles until ready for the analyses to avoid the loss of metal ions due to adsorption.
The crystals were collected and washed using 100% dichloromethane and left to dry overnight under the fume hood to yield 256 mg of cream grainy crystals ready for spectroscopic analyses and structural identification.
It should be noted that the data needs some editing to be ready for the analyses, and that this data is susceptible to the quality of user generated content.
After one night at 4°C, the sections were ready for microscopical analyses.
Library products were ready for sequencing analyses via Illumina HiSeq™ 2000.
In the final step, all aligned output files are merged per individual and are ready for downstream analyses.
The first release of the h3′UTRome library comprises 1,461 human 3′UTRs cloned into Gateway® entry vectors, ready for downstream analyses.
GU apparatus were fixed in phosphate-buffered 10% paraformaldehyde for 4 h and transferred to 70% ethanol until ready for histopathological analyses.
Striatum was dissected on ice and rapidly frozen on dry ice prior to storage at −80°C until ready for monoamine analyses.
This model-based analysis was run with the software STRUCTURE version 2.3.3 (Pritchard et al. 2000), using a batch-oriented web programme package for construction of super matrices ready for phylogenomic analyses (Kumar et al. 2009).
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