For the hyper-variable V6 region, over 45 additional reads are needed for each new phylotype (>97.8% coverage).
These analyses suggest that at least 15 reads are needed to be 95% sure that all four alleles are sequenced.
In addition, we asked how many reads are needed to achieve the same sensitivity as a microarray.
Although only three concordant reads are needed to correct a given error, the optimal result is obtained using 50× of Solexa reads.
Importantly, our nanopore data suggest that very few reads are needed to provide an unambiguous diagnostic identification, despite high individual per read error rates of 10 30 %.
For highly expressed genes, small amounts of sequencing are sufficient, but for the middle and low end of expression levels, it is clear that many reads are needed.
SOAPsv was used to identify structure variation, and at least three paired-end reads were needed to confirm a variation structure in the present study.
To assess how much data (number of reads) is needed to construct the complete transcriptome, different proportions of sequencing data ranging from 5%to100%0% were extracted for both species.
To identify larger numbers of gene-associated SNPs, higher throughput expressed sequence reads are needed to increase coverage and depth and ensure sequence accuracy.
Approximately 150 and 500 reads is needed to detect a heterozygous mutation at a 99%% confidence in a specimen with 20%and10%10 % tumor cellularity, respectively.
