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The raw gene expression counts were normalized using the RPKM (Reads per kb per million reads) value.
The gene expression level was indicated by the log2-transformed RPKM (Reads Per Kilobase per Million mapped reads) value.
The FPKM (Fragments per Kilobase of transcript per Million mapped reads) value was calculated to test the expression levels of the unigenes.
Of the 11068 gene models, 8948 had a FPKM (fragments per kb of exon per million mapped reads) value >1; this value is an indication of significant expression.
Tissue distribution of transcripts was assessed by mapping sequencing reads from each tissue to this global assembly, with an FPKM (fragments per kilobase per million mapped reads) value of >1 taken as confirmation of expression.
The abundance estimation performed on all opsins reported a FPKM (Fragments Per Kilobase of transcript per Million mapped reads) value of at least 300 in the head tissue (Additional file 1: Table S11).
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The New Yorker, May 13 , 1950 P. 20Incidental Intelligence: Across the front of the place of business of an Eight Avenue textile firm reads, "Values at an all-time low".
Frustrated, one consultant sends an e-mail to Mr. Donovan, the Republican candidate for attorney general, which reads: "Values and principles are great.
FPKM (for paired-end reads) and RPKM (for single-end reads) values were generated using the eXpress tool [ 73].
The RPKM (Reads Per Kilobase of exon model per Million mapped reads) values for these five genes range from 3.42e-4 3.42e-4 to 3.27e-3 3.27e-3
Fold changes with respect to RPKM (Reads Per Kilobase per Million mapped reads) values were calculated [ 57].
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