Sentence examples for reads to remove from inspiring English sources

This involved prefiltering the paired-end reads to remove those that did not contain both ends of the pair and screening for Escherichia coli and BAC vector contamination in both Titanium and paired-end reads.

Atlas genome assembly tools [97] were used to process reads, to remove repetitive reads, and to bin overlapping reads before assembling the contigs with the program PHRAP [95].

After trimming reads to remove barcodes and exclude low-complexity or primer sequences, 11,427,212 high-quality 60-bp sequence reads were subjected to an iterative BLASTN analysis pipeline (Fig. 1B).

We also preprocessed the reads to remove the adapter sequence using cutadapt v1.5 [ 43].

Therefore, it was not necessary to trim reads to remove the adapter sequences.

Next, we filter sequence reads to remove low quality sequences and align them to the custom reference genome using GSNAP [ 24], an allele-aware aligner.

To reduce the effect of sequencing errors, we discarded 5′- and 3′-end nucleotides of each read to remove low-quality bases.

The resulting sequences were quality-filtered (Phred quality score cutoff = 20), adapter sequences were removed from the beginning of each read, and 20 bp were trimmed from the end of each read to remove lower-quality bases (Cutadapt version 1.1).

14 bases were clipped off the end of the second read to remove low-quality overlapping ends.

Then, the result of Bowtie is further process to add frequencies of reads and to remove reads with more than 100 aligned loci in the genome.

Prior to de novo assembly, we filtered the read dataset to remove read pairs in which either read had an ambiguous base call (denoted by N) or an average quality score ‹Q25.