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ChimeraScan [ 15] implements an algorithm based on trimming reads to increase fusion detection sensitivity.
The 4 bases CATG were added to the 5' end of reads to increase the specificity of mapping.
Additionally, we only studied genes with a minimum 100 allele-specific reads to increase accuracy in the allelic ratios.
On a related note, I also wonder why the authors did not use long reads to increase their chances of allele-specific mapping of the reads?
In species where nDNA isolation is not feasible, one can increase the number of HTS reads to increase the amount of nDNA sequenced.
Moreover, as high-throughput sequencing is becoming widely used, the haplotyping methods can borrow some partial phase information in sequence reads to increase the accuracy of haplotypes estimated from genotype data [ 32].
Similar(53)
Since the key width of an index (w) here is less than the read length, we are able to apply the mask at all possible starting positions offset from the beginning of the read to increase the number of lookups performed per read and thus further improve sensitivity.
A more recent approach to SNV detection in viral populations, implemented in the program V-Phaser, utilises the covariance between observed SNVs present on the same read to increase sensitivity [ 19].
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We modified the minimum read support parameter from 50 reads to 10 to increase sensitivity.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com