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The preliminary score for an extension is then the sum of these penalties over all gaps and all unitigs in the extension: (2) To produce a final score Pext for each possible extension, we add pext to a 'contig gap penalty', equal to λ times the largest gap size (denoted by g c in Fig. 4c) between two adjacent unitigs, i.e. the expected number of reads to fall in that gap.
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Analysts had expected the reading to fall to 104.5.
To identify inserts from discordant reads, the reads have to fall in exactly the right places (Fig. 1), reducing effective coverage, and many inserts are too small to accommodate a whole read of the 15 polyA inserts we sequenced, four were smaller than 100bp excluding the polyA, and one was only 13bp + 49 As.
Our strict read mapping criteria was meant to identify the most likely candidates; while mirtron reads can be found further from the splice sites in other species, the majority of reads tend to fall in this range.
Although not precocious in most respects, I learned how to read, mostly by myself, at age 4. From earliest childhood, in my tiny town in Brazil, I'd loved to be read to before falling asleep.
I confess: I read fiction to fall in love.
I'll confess — I read fiction to fall in love.
During the next two decades, reading continued to fall and television watching to rise, though more slowly.
The name at the bottom of the note nearly caused the employee reading it to fall from the chair.
If you're a person who reads at night to fall asleep, it's better if you conserve your reading for another time.
Martha, what are you reading to help you fall back asleep?
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com