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Here, we address the first and most expensive step of this process: aligning reads to a reference genome.
Mapping 454 reads to a reference genome.
Bowtie for aligning reads to a reference.
Therefore, mapping reads to a reference genome has two limitations.
The reference bias often occurs when one maps sequence reads to a reference genome.
Mapping sequence assembly: mapping of NGS reads to a reference genome.
Next-generation sequencing projects commonly commence by aligning reads to a reference genome assembly.
Any available mapping algorithm will suffice to map highly identical reads to a reference.
Unspliced aligners may be used to align reads to a reference transcriptome or a reference genome.
Alignments of sequencing reads to a reference can be inaccurate towards the ends of a read.
We demonstrated the feasibility of assembling this genome by mapping reads to a reference genome.
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