The mappability of genes was calculated because if a gene is in or near an area of the genome with high homology to another genomic area, it becomes impossible to assign the genomic origin of an RNA-Seq read stemming from one of the homologous areas.
However, these issues should not affect the overall distributions significantly and, in particular, should not affect the general shape of the graph at all, which this is determined by our assumptions only and not by the sequence the reads stem from.
Our motivation to pursue these reads stems from the striking difference between the number of restriction sites within fully-mapped versus non-mapped reads.
Thus it is not clear from the above result whether the reads stem from the overall baseline transcription, or from high transcription at only a small number of elements.
Such variation can profoundly impact the results of analysis of read stemming from these regions.
But this reading stems from an assumption of continuity between the son and the father, who did pander to evangelical conservatives.
It is unclear whether the lack of deeper reading stems from the obligatory nature of the rating task or whether this also occurs in other contexts because of the time pressure involved in rating.
The PE peaks map algorithmically with negative values due to the different polarity of the read pairs stemming from the circularization in the MP methodology.
Many of the problems students have in reading phylogenetic trees stem from reading them as ladders of progress from left to right (Meir et al. 2007; Gregory 2008).
However, while SMS confers the advantages of higher sensitivity and abrogation of issues stemming from read duplication, the technology has a number of confounding characteristics.
