Low quality reads, reads without the adaptors, reads with polyA sequences and reads without the insert tag where removed.
☆Abundance reflected by normalised reads (reads per million of total miRNA reads, RPM).
The original reads were cleaned by removing adaptor sequences and short reads (reads of < 15 bp).
After excluding low quality reads, reads with 5' primer contaminants, reads without 3' primer, reads without the insert tag, and reads shorter than 18 nt were removed.
Those uniquely aligned reads (reads mapped to unique locations in the reference genome) were retained.
bMapped unique reads, reads that matched the reference genome in only one position.
Only uniquely mapped reads (reads with one single best hit) were kept.
Only the clean reads, reads with adapter detected and trimmed, were used for analysis.
After removing duplicate reads, read error filtering was performed using a rare k-mer filtering approach.
In the no antibody control, ~30,000 (0.08 % of the 0.4 million SAFB1 reads) reads were found.
