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As described by Ruby et al. [13], the observation of both a candidate miRNA and a corresponding candidate miRNA* in a set of reads provides particularly compelling evidence for Dicer-like processing from an RNA hairpin.
We also find that the sequencing depth that we currently employ for standard C.elegans WGS (1 lane of a flow cell, non-paired 100 nucleotide reads) provides sufficient data to query the genome for Bristol/Hawaiian SNPs.
The enrichment of ChIP-seq reads provides probabilistic occupation level as well.
Generation of long (>5 Kb) DNA sequencing reads provides an approach for interrogation of complex regions in the human genome.
A 7% coverage survey with 109.5 bp reads provides 6.25 reads per 10 kb of single copy sequence.
Importantly, the presence of 28 exclusive junction reads provides a strong evidence for the presence of this novel event.
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And one of the president's executive orders reads: "provide incentives for schools to hire school resource officers".
Thus, comparison between the first and (delayed) second visual reads provided an insight into intra-reporter reliability.
The combined reads provided 34× coverage of the genome.
As a result, 29 million aligned reads provided an impressive linear dynamic range (4.37×log10, Fig. 2).
To investigate the quality of sequencing reads provided by NGS platforms, we analyzed whole-genome sequencing data of E. coli DH1 ME8569 strain.
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