Your English writing platform
Discover LudwigExact(1)
The origin of MP reads oriented in FF/RR direction, which make up ~38% of the total MP data, is less obvious.
Similar(59)
Applications use different kinds of data that must be managed according to different purposes: some data collections are read oriented with few writes; other data is modified continuously, and it is exploited by non-concurrent read operations.
I was reading Orient by Christopher Bollen, one of those big fat summer mystery books, and I had zipped through the first third of the book with no problem.
The chapter openly permitted a reading oriented around pauses — for reflection or rumination, perhaps, but also for refreshment or diversion.
Investigating the mapping orientation within CDSs revealed that 97% of the reads are oriented according to the coding strands and thus agree with the presence of mRNA transcripts.
The finding, that 30.6% of the genic reads were oriented in antisense to the transcript of origin may suggest a role for antisense transcription in generating endo-siRNAs.
More than 50% of intergenic reads are oriented on the sense strand relative to neighboring genes and most likely represent the transcription boundaries of predicted sponge gene models, including 5' and 3' untranslated regions [ 32].
In fact, the distribution of wrongly oriented reads reveals that previously proposed mechanism only constitute a part of these, and instead the majority of these reads arise from fragments containing the same sequence on both ends with one end being an inverted version of another.
It is not uncommon to have PE oriented reads (i.e. FR) in MP libraries (c.f. mate pair library sample preparation guide), but the occurrence of FF/RR reads is more difficult to explain.
We also found that the wrongly oriented reads did not cause more miss-assemblies than expected.
First, inverted repeats are known to generate chimeras in WGA protocols, the extent of which we assessed by recording the number of wrongly oriented reads in the samples.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com