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The remaining 1% (2491 reads) of the data ambiguously map to more than one transcript at the high stringency used, although these ambiguous matches are usually simple repeats or sequences of low-complexity.
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The filtered reads of the original data sets were randomly pruned to data set sizes of 10 million, 4 million, and 1 million reads.
Moreover, these numbers were also rather variable between "forward" and "reverse" orientation GS FLX reads of the amplicon (data not shown).
This can be counted directly from the NGS short reads of the metagenome data, without the need of full or partial sequence assembly.
During gene sequence classification, the short reads of sequence data commonly generated by modern sequencers can be in either forward or reverse-complement order.
Three main groups of tag sequences were observed from the uniquely mapped reads of EXPRSS data sets.
Due to the differing abilities to read of the study population the data collection was carried out by interview.
Therefore, the number of read out pixels is reduced and the collection of photons occurs simultaneously with the read out of the data of previous pixels.
The experimental error bars were derived by fitting a linear function to the backward events and reading deviations of the data from the fitting function (Fig. 6(c)).
This cut-off is adjustable according to the median read depth of the data and the diagnostic purpose.
This enthusiasm rests on a very selective reading of the data.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com