Sentence examples for reads of minimum from inspiring English sources

Each base position also required at least two sequence reads, of minimum Phred20, that were in agreement with the consensus sequence (i.e., no high-quality discrepancies).

All the corresponding raw sequence data [SRA Study ERP000190, Manske et al. (2012); Robinson et al. (2011)] consists of paired end reads of minimum length 54 bp and was mapped to the 3D7 P.falciparum reference genome (v3.0) using smalt (www.sanger.ac.uk/resources/software/smalt/).sanger.ac.uk/resources/software/smalt/

Trimmed reads of V. minimum were mapped to two reference plastomes of Vitis vinifera (NC_007957) and Fagopyrum esculentum (NC_010776).

The black line depicts the cumulated amount of bases covered by reads of a minimum size as shown on the x-axis.

SNVs and InDels ranging from 1 to 5 bp were sorted and called at minimum reads of 10.

a contigs were selected with minimum length of 300-bp and minimum mapped reads of 50.

Using SHORE's scoring matrix approach optimized for heterozygous SNP detection and stringent filtering (requiring uniqueness of reads, minimum 10× coverage, minimum 20% allele frequency, SHORE SNP quality score >25), 354,080 SNPs could be identified (Supporting Information, Table S2).

Finally, SNPs, which had a Phred score higher than 30 (i.e. their base-calling accuracy is larger then 99.9%), and a high-quality read coverage of minimum three, were filtered using a custom script.

Several sequences had very low number of mapping reads (minimum of 2; Additional files 2 and 3) and might be mapping artefacts, but were still considered.

Prinseq-lite 0.20.04 (Schmieder and Edwards 2011) was used for quality control and filtering of reads (minimum read length of 50 nt, minimum average base quality of 25, maximum ambiguous nt (N) of 1) after which the data were used for SSR mining.

miRCat was running with minimum sRNA abundance of 5 reads, minimum size of 20 nt, maximum size of 24 nt and maximum number of 16 genome hits.