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For the first time in Test cricket the score book entry reads: "Match drawn with scores level".
Since some reads match multiple isoforms, their isoform of origin cannot be established unambiguously.
In BWA, if reads match several genomic regions, one genomic location is randomly chosen as default (Li and Durbin, 2009).
Not unexpectedly, only ~218 000 of these unique reads match the limited available genomic barley BAC sequences.
U0 reads match unique genomic regions with zero mismatches, U1 reads align to unique regions with one mismatch, and U2 reads align to unique regions with two mismatches.
The fact that the hypothetical genes are highly expressed and the RNA-Seq reads match nicely to the gene models suggest that they are real genes.
Similar(39)
The gene expression profiling analysis was based on the number of reads matching the exon regions.
Eighteen reads matched Yersinia species.
A low percentage of the clone reads matched annotated m/t/r/sn/snoRNA.
In seedling, greater than 30% of the ∼21-nt ∼21-ntant redundantched to known mireads
Several reads matched both commensal and pathogenic organisms with equally strong identity percentages.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com