Taxonomic classification of non-human nanopore reads identified using MetaPORE.
Fifteen known repeat element-containing reads identified by RepeatMasker were not identified by RepeatScout.
These reads comprise 93% of all microbial reads identified at the species level in the sample.
The fraction of endogenous Illumina reads identified is less sensitive to the mapping strategy.
In addition these GS FLX reads identified 22,193 exon junctions not yet in RefSeq.
Additionally, any reads identified as containing residual adaptor sequence were removed before transcriptome assembly.
Only uniquely mapping reads were retained and reads identified as PCR duplicates were discarded.
This de novo assembly of the expressed reads identified 121 assembled contigs in the targeted interval.
Thereby, analysis of 147 204 502 reads identified 80 genes differentially expressed in CKD patients.
The number of sRNA reads identified at each step of the miRNA prediction pipeline is summarized in Table 1.
Bioinformatics analyses of 93,526,915 reads identified 135 conserved miRNA, belonging to 22 miRNA families in the olive.
