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Sequences and reads for multiple species were retrieved from the NCBI Nucleotide Database http://www.ncbi.nih.gov/sites/entrez?db=nuccore and Traces Database http://www.ncbi.nih.gov/Traces.nih.gov/Traces
(i) We generated low-coverage Illumina whole genome shotgun sequencing reads for multiple individuals of cacao (Theobroma cacao) and related species.
Furthermore, the number of reads for multiple mature miRNA strands derived from the same precursor differed dramatically, which indicated that the hydrolysis process mediated by Dicer and its associated proteins might not be very precise and the miRNA stability might be determined by the terminal nucleotide residues.
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For each read, the best alignment to the genome was kept in the output, with the exception of chimeric reads, for which multiple alignments were retained.
Finally, instead of single genomes the comparison sequence files can consist of multiple DNA sequences in FASTA format (next-generation sequencing reads for example) or multiple protein sequences in FASTA format (a custom collection of bacteriophage proteins for example).
Here we note that the assembly process, by default, may split reads for use in multiple c-isotigs.
Here we present KvarQ, a software that enables rapid screening of short sequence reads for mutations at multiple nucleotide positions of interest.
The rate at which multiple reads for a gene coalesce into a single contig is a function of read length.
We also consider an estimator which discards multiple reads for a SNP and individual, by randomly sampling one read from those available.
In order to guard against this problem, we should distrust any reads for which there exist multiple possible alignments whose distance from the genome is less than some safety margin ε.
In this section, we will describe our algorithm, Meta-IDBA, for assembling reads from multiple genomes of subspecies in different species.
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