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A significant fraction (∼11%) of the metagenome sequence reads exhibit reasonable nucleotide identity (80 99%) to the available genomic sequence (∼2200 ORFs) of Metallosphaera sp. str.
The results, presented in Table 1, indicate that only 13% of the reads find an exact match, and 85% of the reads exhibit variants or sequencing errors.
The pair distance distribution plot can be used to judge whether the matched paired-end/mate-pair reads exhibit the correct distance distribution.
There may be other plausible models, for example ones where allele reads exhibit clustering such that observed homozygosity is higher (when depth exceeds one) than in the random reads case, but which still have K = ½ when k = 1.
Unless specific enzymatic treatments are used (e.g. as part of the library building protocol; see [ 22]), aDNA reads exhibit high rates of mismatches at 5'-termini due to the prevalence of post-mortem cytosine deamination at overhanging ends.
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The longest contig was 5,049 bp in length and exhibited sequence homology with peroxidase 12; the deepest contig had 1,795 reads exhibited homology with unknown proteins from Picea species.
At the TAZ target site, 54% of DNA-sequencing reads exhibited short indels suggestive of Cas9 activity.
The remaining 57% of reads exhibited one- to three-nucleotide variations from the reference EBV miRNA sequences in miRBase.
Additionally, about 0.3% of the Enz+pyrosequencing unmapped reads exhibited sequence identity to an unknown member of the Bacillus genus.
In addition to the length variations, more than 50% of the EBV miRNA deep sequencing reads exhibited one- to three-nucleotide mismatches to the reference sequence of EBV miRNA.
20.5% of miRNA reads exhibited 3' end variability compared to only 0.8% of reads for 5' variability.
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CEO of Professional Science Editing for Scientists @ prosciediting.com