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If a control sample is available, then it is further required that there is at least an m-fold enrichment (default 5-fold) of reads in the ChIP sample over the control within the same boundaries, and the data are recommended to be normalized into numbers on a per-million reads basis.
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It may try to charge admission, perhaps pennies per page on a "pay as you read" basis if it can compete with the free headline services that will proliferate on the Web.
However, on a per read basis both libraries had quality scores greater than 37 for most reads (Additional file 2: Figure S2).
Though Pacific Biosciences data are more expensive on a per base or per read basis than for MiSeq, for the experiments described herein, reagent costs are similar.
Transcriptome-sequencing clean reads is basis for assembly and following analysis (the data are archived at the NCBI Sequence Read Archive under accession number SRA058577; http://ncbi.nlm.nih.gov/sra).nih.gov/sra
To this end, iterative filtering steps were employed to remove 80-86% of the reads on basis of alignment to rRNA/tRNA (bacterial, archaeal and eukaryotic origin), to phiX, and to the Illumina adaptor sequences (Additional file 3: Table S2).
First, the quality of Sanger-sequencing is currently the highest on a single read basis.
Samples were considered positive for brucellosis if they were positive for mRBPT and CFT on the serial reading basis.
Successful application of this approach may enable researchers to better understand the confidence of taxonomic assignments on a read-by-read basis as well as across taxonomic ranks.
The alphabetFrequency function summarizes nucleotide use over all cycles, on a per-read basis or over the entire set of reads.
In addition to this, significant development of new parsing routines took place in an Avogadro plugin to read in basis sets and electronic structure output in order to calculate molecular orbital and electron density grids.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com