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The devices were polled approximately once per second, and whenever the readout value changed, the time and sky brightness measurement were recorded to a file.
Mean pixel intensity was taken as readout value.
The stable TLDs were then used in a dose-verification system, and the dose calibration curve was found to be suitable for converting the TLD readout value into an accurate dose.
Our implementation of the SDRS algorithm includes a typical sigmoidal dose response model for one-site compound:target interaction, where Y is the assay readout value, X is the dose and the four unknown parameters correspond to minimal response (A), maximal response (B), EC50 (C) and the Hill slope (D).
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The firefly-luciferase readout values were first normalized using the corresponding renilla-luciferase readout values.
We obtained readout values between 0 and 0.587.
A t-test on the photoluminescence readout values of the original sample set showed a p-value below 0.05 when comparing the RR and PP MScl samples (Table 3).
Readout values were normalized to the values of the loading control (18S rRNA).
Correction factors are important for converting TLD readout values from phantoms into the absorbed dose in water.
All of the luciferase values were normalized to the Renilla luciferase readout values and expressed as x-fold induction relative to that of Ad-LacZ infected cells.
After analyzing the TLD readout values, we used the determination coefficient (R) of the linear fitting curve to assess the linearity of the dose-response curve.
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