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Kinetics studies were performed using real-time PCR as a readout to quantify this change in IL-6 mRNA levels.
Leu1 encodes a cytosolic Fe-S protein that was being used as a readout to quantify cytosolic Fe-S cluster biogenesis.
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In addition, phosphorylated STAT1 dimer and IRF-1 were examined as readouts to quantify the level of priming effect [ 31].
We took advantage of this sensitive LI-COR-based dual-color readout to simultaneously quantify the extent of label incorporated at specific azF positions on CCR5 and the total receptor level.
We did, however, implement direct state tomography of the register qubits for certain states to compare readout methods and to quantify errors (see Discussion and Methods), but we found this to be generally unreliable for arbitrary states.
We used automated image analysis to quantify BiFC fluorescence readouts within individual cells in an unbiased manner, although other methods are also possible (Morell et al., 2007).
We killed mice of both cohorts after 4 days of treatment and measured spleen weight as readout of its degree of infiltration with leukemic cells, to quantify response to treatment.
Clomeleon was functional after chronic expression and provided non-invasive readouts of intracellular chloride concentration ([Cl](i)) in brain slices, allowing us to quantify age-dependent declines in resting [Cl](i) during neuronal development.
To quantify this concept, we define memory orthogonality between two pathway readouts Y1 and Y2 to be: Memorth(M;(Y1,Y2)) ≡ (I(M;(Y1,Y2))-max(I(M Y1),I(M Y2)))/min(I(M Y1),I(M Y2)), where M is cell history and I is mutual information.
To quantify the ability for regulatory volume control, we used hydraulic conductivity (Lp) as readout and demonstrated a role of the cytoskeleton in protoplast swelling.
The described multi-parametric method is a rapid way to quantify levels of endogenous β-catenin and identify non-specific cytotoxic conditions that result in artifactual readouts.
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