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To develop a CETSA assay for AR, we first investigated AR thermostability using quantitative western blot as a readout to detect the amount of soluble thermostable protein following heat shock, a methodology henceforth referred to as CETSA Classics.
In our model, expression of a dual drug resistance marker, driven by an NF-κB-dependent minimal promoter, provides a selective and highly sensitive scheme with a quantitative readout to detect biochemical agents with pro-or anti-inflammatory properties.
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We applied an image processing analysis, a clustering analysis method and statistical analyses of intensity readouts to detect host factors involved in HCV infection.
Testing the model (Predicted concentrations ~ Membrane readouts) fitting to detect biases and to check the overall quality of the distribution also gave promising results.
In summary, we designed an optical readout system able to detect changes of the pH value by the immobilization of CdTe QDs in a PA hydrogel.
To corroborate that the Wnt reporter provided a consistent Wnt-activated cell readout, antibody staining to detect cells harboring nuclear localized β-catenin was performed.
To our knowledge this is the first study evaluating the ability of this novel imaging modality comparing quantitative fluorescence readout to synovitis detected by MRI and US.
The reasons for the contradictory results on clinical signs in these studies were attributed to selection of the patients, design of the study and the readouts, the power to detect a clinical effect and the use of a combination of estrogens and progestagens, which may obscure effects of estrogen alone [ 5, 56].
The readout system was able to detect the resonance frequency, fsensor, a function of inductance and capacitance as described in the Methods section10,17.
However, microdroplet methods come with additional challenges, including the need for highly sensitive optical readout systems in order to detect low levels of signal (e.g fluorescence) in small droplets moving very fast.
The accelerometer, equipped with this readout scheme, should be able to detect the inertial displacement of a platform with a root-mean-square noise less than 1 nm, integrated over all the frequencies above 0.01 Hz.
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