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Readout sequence begins with trigger signal from ASICs in first SSD layer.
Four k-space segments were acquired using a segmented readout sequence called TRICKS [18].
Remove offset using the overscan linear or quadratic offset: stretch out the readout sequence in time and fit a second order polynomial over the overscan; 4.
Perform a Fourier transform of the readout sequence of the background for each channel, remove high frequency spikes by thresholding, and subtract from data; 6.
Each channel included a charge-sensitive preamplifier, a fast CR-RC shaping amplifier followed by a discriminator that generated a trigger signal for the readout sequence, and a slow CR-RC shaping amplifier followed by a sample-and-hold circuit for pulse-height analysis.
Each channel includes a charge-sensitive preamplifier, a fast CR-RC shaping amplifier followed by a discriminator generating a trigger signal for the readout sequence, and a slow CR-RC shaping amplifier followed by a sample-and-hold circuit for pulse-height analysis.
Similar(52)
Then, the cell is interrogated bit by bit using oligonucleotides complementary to the readout sequences.
To do that, oligonucleotides complementary to each transcript are flanked by readout sequences corresponding to the different bits.
This pool, which may comprise 100,000 oligos or more, is hybridized to the mRNAs in the fixed cells, "convert[ing] each cellular RNA into a combination of readout sequences," Zhuang explains.
These interactions may be broadly classified into base readout (sequence-dependent interactions with DNA bases via the major or minor groove) and shape readout (detection of global or local variation of the canonical DNA shape; Rohs et al., 2010).
These technologies will provide an inexpensive, genome-wide sequence readout as an endpoint to applications ranging from chromatin immunoprecipitation, mutation mapping and polymorphism discovery to noncoding RNA discovery.
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