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The readout script is synchronized with the pixel clock of the microscope.
A low level camera readout script was written and a customized driver is used for optimized performance.
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The in-house developed script and spectral analyses allowed the readouts to be presented either in time domain or in frequency domain.
The Matlab script and the spectral analyses allow display of the readout in time domain or in frequency domain respectively, thereby providing deeper understanding of the effects produced by the compounds.
Images taken with the sCMOS camera was analyzed with a previously published sCMOS-specific script which takes into consideration sCMOS-intrinsic-pixel-dependent readout noise and achieves optimal precision at the theoretical limit (Huang et al., 2013).
The variety of readouts that can be obtained along with the in-house developed analyses script offers a powerful setup for performing cardiac toxicity evaluation by researchers from both academics and industry.
The continuous acquisition of VEEL spectra was realized by a self-written script in Gatan DigitalMicrograph with an exposure time of 0.5 s, a CCD readout area of 512 × 2048 pixels and a pixel binning of 2. With this, a time resolution of 1.5 s per spectrum could be achieved.
"Timer, digital readout.
"0 Min," read the blinking L.C.D. readout.
"Data program readout: number fourteen ninety-two per centH2SOSOO".
It is an electronic readout level that replaces the bubble display with a digital readout panel.
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