The engineers at Hario not only put a timer next to the readout screen, but added a couple of hidden features that address the specific needs of brewing coffee.
Phenotypic cell-based screens have led to the identification of structurally diverse inhibitors of cancer cell invasion [9] and we developed a positive readout screen of this type that eliminates actutely cytotoxic compounds [10] to identify the motuporamines [10], [11], strongylophorine-26 [13], [13], avinosol [14] and the neopetrosiamides [15].
The phones, for example, didn't used to have little readout screens, but now they all do, and the ringtones have changed besides.
We used the duration of this growth arrest as readout to screen for timing regulators of the stress response.
To identify the cognate neuropeptide(s) activating ASEL neurons, we used ASEL activity as readout to screen a number of neuropeptide gene mutants.
Upregulation of miR-203 turned out to be an efficient readout to screen for chemosensitizing substances, leading to the identification of the HDACi mocetinostat as a candidate.
The authors then used Ucn3 as a readout to screen for factors that reverse beta cell dedifferentiation and identified a small molecule inhibitor of TGFbeta receptor I (Alk5) that showed protective effects on beta cells and restored mature beta cell identity.
Although it serves as an easy readout for screens, cell models, and neuropathology, the exact role of aggregates in the neurodegeneration observed in polyQ diseases is still under debate.
Using a simple growth-based functional readout, we screened a library of 106,281 small molecules and identified two new inhibitors and three new activators of the mammalian K2P channel K2P2.1 (KCNK2, TREK-1).
The screen readout is independent of the function of the test protein, proteolytic resistance, solubility or propensity to aggregate indiscriminately, and is thus dependent solely on the overall stability of the test protein.
As a readout for the screen, we used a reporter construct containing the cis-regulatory promoter region (5.8 kb containing an AR enhancer) of the prostate-specific antigen (PSA) gene, which is highly expressed in the normal prostate epithelium and in prostate tumors [28], [29].
