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However, the reporter gene knockin is predicted to result in minimal disruption of the native cis regulatory sequences that govern c-Myc transcription so that the reporter gene will provide an accurate readout of endogenous c-Myc activity.
DsRed provides a stable and cumulative readout of endogenous hsp70 induction: Whole-mount antibody labelling with hsp70 (green) (A-D) and DsRed (E,F) in non-heat shocked control (A,C,E) and heat shocked (B,D,F) zebrafish embryos at 29 hpf (A,B), 39 hpf (C,D) and 48 hpf (E,F).
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We measured circadian regulated asexual conidiation (banding) as a reliable phenotypic readout of the endogenous cellular clock [29].
Circadian regulated conidiation (banding) serves as an accurate phenotypic readout of the endogenous cellular clock and is measured with the race tube assay [1].
This sensor responded to defined perturbations of the BMP-Smad pathway (Supplementary Figure 7), indicating that it serves as reliable readout for endogenous signaling.
In fact, when PR-dependent transcription peaks, as measured by RT-qPCR of endogenous gene readouts (via mRNA levels, as in Figure 5B) or using reporter genes, PR protein levels are virtually undetectable [ 39].
This technique, based on the magnetic properties of endogenous deoxyhemoglobin (deoxyHb), enables rapid readouts of the dynamic balance between circulating paramagnetic deoxyHb and diamagnetic oxyHb.
In this study, we performed the widely used analyses of endogenous phosphorylated Akt S473 as functional readout for mTORC2 activity.
Utilization of a short-lived fluorescent reporter protein or mRNA FISH of endogenous target genes will be required to bring an improved dynamic readout of gene expression into the multiplex analyses.
The described multi-parametric method is a rapid way to quantify levels of endogenous β-catenin and identify non-specific cytotoxic conditions that result in artifactual readouts.
The word is an amalgam of endogenous, meaning made by the body, and morphine.
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