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Screening of an enzyme activity in individual intact cells, typically using cell survival for essential reactions, or flow cytometry (FACS; fluorescence-activated cell-sorting) if a fluorescent readout of activity is available, is a particularly efficient approach to library screening, but it also has particular restrictions.
We revised this paragraph to emphasize that the On parasol cells provide a good readout of activity in the rod bipolar pathway.
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This organization afforded us a fluorescent readout of Cre activity, with activity in 100% of cells correlating to roughly 50% red fluorescent cells [24].
It is shown that phosphorylation of DNA-PKcs at Ser2056 indicates the readout of activation of the catalytic activity.
According to one view, these subjective experiences are illusory (Wegner, 2002), and the "conscious will" is merely a retrospective inference, rather than a direct readout of brain activity associated with action selection or action generation.
We next asked if Ttk69-bound and Lmd-bound CRMs have differential activity, using chromatin state as a readout of enhancer activity.
Since LKB1 is likely to be the major upstream kinase for LKB1 in islets (I. Leclerc and G. A. Rutter, unpublished results), AMPK activities were also measured as a further readout of LKB1 activity.
Thus, all cells of Z/AP and Z/EG mice are designed to exhibit a binary readout of Cre activity, expressing β-galactosidase by default and activating expression of hPLAP or EGFP respectively upon exposure to Cre.
In the process of generating a Cre-mediated reversible switching line with a fluorescent readout of Cre activity (Fig. 1A), we observed a high level of ligand-independent mCrem activity.
The HFRs from all four clusters were tested for enhancer-blocking activity in a human cell line using the number of neomycin resistant colonies formed as a readout of enhancer activity.
In this study our readout of biological activity was chemotaxis, which is the final downstream function of CXCR3 activation and may be attained by several signalling pathways.
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