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Each readout cell is equipped with an amplifier, comparator, 8-bit counter and 16 count-registers.
Each readout cell contains a low noise charge-sensitive amplifier, three-stage pulse shaping amplifiers and a comparator circuit.
Each readout cell comprises this front-end and digital readout circuitry, and has a static power consumption of about 60 μW.
For luciferase readout, cell lysates were prepared (Glo-Lysis buffer, Promega) and 50 μl lysate mixed with 50 μl Ren-Glo luciferase solution (Promega) at RT and activity (relative light units, RLU) measured after 10 min.
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The SPD is made of hybrid silicon pixel detectors with a total of 9.8×106 readout cells.
In a prototype ASIC, 36 readout cells were implemented by TSMC 0.25‐μm CMOS process.
The chip is a matrix of 32 columns each containing 256 readout cells.
A small circuit composed of 32 single pixel readout cells was implemented in a 130 nm CMOS process.
The high energy deposits in the calorimeter readout cells have been used to validate the signal shape reconstruction from calibration predictions at a level better than ∼2%.
The electronic readout, cell-sensor impedance is displayed as arbitrary units called cell index, which is defined as Rn-Rb/Rb, with Rn = cell-electrode impedance of the well with the cells and Rb = the background impedance of the well with the media alone.
In further experiments, 10 μM LPA was chosen as the preferred dose for all the cells, and readout for cell migration was set at 24 h for the E10 cells, 48 h for the SCC-9 cells, and 17 h for the D2 cells.
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