This aspect is important for proper differential read-out analysis, only sensors with equal mechanical properties should be utilized for differential readout.
Important technical considerations include the choice of silencing reagents, experimental systems, assay readout and analysis methods.
The entire device is fully automated from optical alignment to sample injection, readout and analysis.
This paves the way to broad use of the assay, and enables increased throughput via automated assays, readout and analysis, as we have done.
Most of the studies tested the hORFs function in an array (well-based) format [ 43- 45] that simplifies the readout and analysis of the results.
To determine the timing conditions that minimise the protective effect of PTX, we used as readout the analysis of central tumour necrosis caused by ZD6126 (Taraboletti et al, 2005).
We have recently developed an automated miniaturized drug screening assay based on our EHT technology and neonatal rat cardiac myocytes, which appears to combine some of the advantages of a relatively intact 3D cardiac tissue, availability at large numbers, robustness and high-content readout, particularly analysis of contractile force and kinetics [ 7, 16, 20].
The negative readout from this analysis relieved safety concerns to use ipNPCs for cell transplantation in the future.
TFE3 post-translational modifications and its subcellular localization could be an important readout for the analysis of FLCN function and the function of the FLCN/FNIP1/FNIP2/AMPK complex.
For this reason, we here utilized changes in NHE1 activity as the readout for the analysis of the role of a particular signal transduction pathway in transformation in the dissection of the early signaling events occurring up-stream of NHE1 activation during E7-dependent transformation.
