For example, one could generate fluorescent reporters to readout pathway activity, which could allow for more targeted drug screening.
We revised this paragraph to emphasize that the On parasol cells provide a good readout of activity in the rod bipolar pathway.
As readout for activity-based methods, scintillation [ 11] and fluorescence polarization [ 16] have been used among others [ 14, 15].
This organization afforded us a fluorescent readout of Cre activity, with activity in 100% of cells correlating to roughly 50% red fluorescent cells [24].
It has been shown that interference with physiological responses may affect the immune activity readout e.g PPO activating enzymes, but how these responses are coordinated and regulated is not yet known.
According to one view, these subjective experiences are illusory (Wegner, 2002), and the "conscious will" is merely a retrospective inference, rather than a direct readout of brain activity associated with action selection or action generation.
Since LKB1 is likely to be the major upstream kinase for LKB1 in islets (I. Leclerc and G. A. Rutter, unpublished results), AMPK activities were also measured as a further readout of LKB1 activity.
Induction of HO-1, an enzyme decomposing heme to biliverdin, iron, and CO2 and accounting for many beneficial outcomes on Nrf2 activation (e.g., [ 22- 24]), served as readout for Nrf2 activity.
Thus, all cells of Z/AP and Z/EG mice are designed to exhibit a binary readout of Cre activity, expressing β-galactosidase by default and activating expression of hPLAP or EGFP respectively upon exposure to Cre.
In the process of generating a Cre-mediated reversible switching line with a fluorescent readout of Cre activity (Fig. 1A), we observed a high level of ligand-independent mCrem activity.
