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A total of 2,436 readings were conducted.
Further readings were conducted at 24, 48 and 72 h after the addition of drug.
Absorbance readings were conducted with a spectrophotometer at a wavelength of 570 nm, using KC4 v3.4 and KC4 Signature programs.
Negative controls were run, and comparisons to microsatellite readings were conducted to test for possible contaminations between neighbouring DNA wells.
The readings were conducted with the SYSMEX K-1000 Reader, a product of San-Tong Instrument Co. (Taipei, Taiwan).
Additional readings were conducted to better understand the interactions of factors during which uncertainty was identified as one of several factors mentioned during interviews and focus group discussions.
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Experimental tests, with simulated biases on the encoder׳s readings, are conducted and the obtained results are presented.
[35] The interpretation of spectrophotometry readings was conducted according to the updated procedure described by Washington and co-workers.
And then, CCK-8 (10 μL) was added to each well and the samples in the 96-well plate were further incubated for a further 4 h before the absorbance readings, which were conducted at 450 nm using FluoStar Optima microplate reader (LUOstar OPTIMA, BMG Labtech GmbH, Germany).
Three readings of each project were conducted for simultaneously filling instruments 1 and 2. After reading the 16 projects, data from the checklist were analysed according to the total presence (Y = yes) or absence (N = no) of the elements related to the strength-of-evidence of effectiveness (Jiménez et al. 2014), according to the content of each project.
Furthermore, the readers were highly specialized musculoskeletal radiologists, and training and calibration sessions were conducted prior the readings.
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